Nuclear traffic of influenza virus proteins and ribonucleoprotein complexes. Mutational analysis of the influenza virus cRNA promoter and identification of nucleotides critical for replication. Promoter elements in the influenza vRNA terminal structure. Functional analysis of the influenza A virus cRNA promoter and construction of an ambisense transcription system. Absence of detectable capping and methylating enzymes in influenza virions. Influenza viral mRNA contains internal N6-methyladenosine and 5'-terminal 7-methylguanosine in cap structures.
Nonviral oligonucleotides at the 5' terminus of cytoplasmic influenza viral mRNA deduced from cloned complete genomic sequences. Cap and internal nucleotides of reovirus mRNA primers are incorporated into influenza viral complementary RNA during transcription in vitro.
Both the 7-methyl and the 2'-O-methyl groups in the cap of mRNA strongly influence its ability to act as primer for influenza virus RNA transcription. Nucleic Acids Res. The active sites of the influenza cap-dependent endonuclease are on different polymerase subunits. EMBO J. Influenza A virus and the cell nucleus. Functional association between viral and cellular transcription during influenza virus infection.
Rev Med Virol. Genes Dev. Influenza virus NS1 protein alters the subnuclear localization of cellular splicing components. J Gen Virol. The influenza virus panhandle is involved in the initiation of transcription. Recombinant influenza virus polymerase: requirement of both 5' and 3' viral ends for endonuclease activity. Polyadenylation sites for influenza virus mRNA. Polyuridylated mRNA synthesized by a recombinant influenza virus is defective in nuclear export.
Mol Cell. Influenza virus gene expression: control mechanisms at early and late times of infection and nuclear-cytoplasmic transport of virus-specific RNAs. In vitro dissection of the membrane and RNP binding activities of influenza virus M1 protein. Nuclear dynamics of influenza A virus ribonucleoproteins revealed by live-cell imaging studies. Biol Lett. The cytoplasmic tail of the influenza A virus M2 protein plays a role in viral assembly. Influenza a viruses with mutations in the m1 helix six domain display a wide variety of morphological phenotypes.
An influenza virus containing nine different RNA segments. Evidence for segment-nonspecific packaging of the influenza a virus genome. Replication of the influenza virus genome. Selective incorporation of influenza virus RNA segments into virions. Free to read. The role of the influenza A virus panhandle structure formed from the 3'- and 5'-terminal nucleotides of virion RNA segments was studied in both an RNA polymerase binding assay and an in vitro transcription assay. Despite recent indications that promoter activity is simply a function of the 3'-terminal sequence of virion RNA, our results show that both 3'- and 5'-terminal sequences are involved in the initiation of transcription.
We propose a new model for the initiation of transcription which has implications for the mechanisms by which influenza virus transcription, replication, and polyadenylation may be regulated in the infected cell.
Read article at publisher's site DOI : Free to read at jvi. Viruses , 13 5 , 28 Apr Biomolecules , 11 1 , 19 Jan Nat Rev Microbiol , 19 5 , 25 Jan RNA Biol , 18 4 , 22 Oct Viruses , 12 9 , 11 Sep To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.
J Virol , 70 2 , 01 Feb J Gen Virol , 76 Pt 9 , 01 Sep Cited by: 27 articles PMID: J Gen Virol , 74 Pt 7 , 01 Jul Cited by: 61 articles PMID: Acta Virol , 44 5 , 01 Oct Cited by: 12 articles PMID: Curr Top Microbiol Immunol , , 01 Jan Cited by: articles PMID: Contact us.
Europe PMC requires Javascript to function effectively. Recent Activity. Search life-sciences literature Over 39 million articles, preprints and more Search Advanced search. Corresponding author. Address correspondence to Frank T. Vreede, ku. Received Mar 5; Accepted Jun This is an open-access article distributed under the terms of the Creative Commons Attribution 3.
This article has been cited by other articles in PMC. Open in a separate window. Fig 1. Fig 2. Fig 3. Footnotes Published ahead of print 17 July The influenza virus RNA synthesis machine: advances in its structure and function. RNA Biol. Towards an atomic resolution understanding of the influenza virus replication machinery.
Gene 8 — [ PubMed ] [ Google Scholar ]. Author information Copyright and License information Disclaimer. Copyright notice. This article has been cited by other articles in PMC. Images in this article Image on p. Image on p. Barbosa E, Moss B. Purification and physical properties.
J Biol Chem. Characteristics and substrate specificity. Nucleic Acids Res. Synthesis of the templates for influenza virion RNA replication in vitro. Transcription of the influenza ribonucleic acid genome by a virion polymerase.
Optimal conditions for in vitro activity of the ribonucleic acid-dependent ribonucleic acid polymerase. Cap-recognizing protein of influenza virus. Identification of the cap binding protein of influenza virus. Cap and internal nucleotides of reovirus mRNA primers are incorporated into influenza viral complementary RNA during transcription in vitro. Both the 7-methyl and the 2'-O-methyl groups in the cap of mRNA strongly influence its ability to act as primer for influenza virus RNA transcription.
Structure of the host-derived sequences present at the 5' ends of influenza virus mRNA. The 3' and 5'-terminal sequences of influenza A, B and C virus RNA segments are highly conserved and show partial inverted complementarity. Nonviral oligonucleotides at the 5' terminus of cytoplasmic influenza viral mRNA deduced from cloned complete genomic sequences.
Single cell assay of a transcription factor reveals a threshold in transcription activated by signals emanating from the T-cell antigen receptor. Genes Dev.
Photochemical cross-linking of influenza A polymerase to its virion RNA promoter defines a polymerase binding site at residues 9 to 12 of the promoter.
J Gen Virol. Characterization of influenza virus RNA complete transcripts. J Biochem. Complexes of Sendai virus NP-P and P-L proteins are required for defective interfering particle genome replication in vitro. Genomic RNAs of influenza viruses are held in a circular conformation in virions and in infected cells by a terminal panhandle.
Determination of influenza virus proteins required for genome replication. Overexpression of the influenza virus polymerase can titrate out inhibition by the murine Mx1 protein.
Viral RNA polymerases. The gene structure and replication of influenza virus. Annu Rev Biochem.
0コメント